商品名称：Lumit™ Immunoassay Cellular System - Starter Kit
The Lumit™ Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect any phosphoprotein, total protein or small molecule of interest, provided that appropriate primary antibodies (supplied by the user) are available.
To save you time, we have evaluated a list of common targets using commercially available primary antibodies. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.
| Cellular Target | Signaling Pathway | Total Protein Protocol | Phosphorylated Protein Protocol | Lumit Immunoassay Cellular System | Lumit Cat.# | Application Note |
|---|---|---|---|---|---|---|
|
4E-BP1 (Human) |
mTor | No | Yes (Ser 65) | Set 2 | W1331 | Download |
|
AKT (Human) |
PI3K/mTOR/AKT | Yes | Yes (Ser 473) | Set 1 | W1201 | Download |
|
BCL6 (Human) |
B-cell signaling | Yes | No | Set 1 | W1201 | Download |
|
β-catenin (Human) |
WNT | Yes | Yes (Thr 41/ Ser 45) | Set 2 | W1331 | Download |
|
BTK (Human) |
B-cell Receptor (BCR) | No | Yes (Tyr 223) | Set 1 | W1201 | Download |
|
c-Jun (Human) |
JNK | No | Yes (Ser 63) | Set 1 | W1201 | Download |
|
CREB (Human) |
PKA/CREB | Yes | Yes (Ser 133) | Set 1 | W1201 | Download |
|
ER (Estrogen Receptor - Human) |
Estrogen | Yes | No | Set 1 | W1201 | Download |
|
ERK1 (Human) |
ERK | No | Yes (Thr 202) | Set 1 | W1201 | Download |
|
IκBα (Human) |
NF-κB |
Yes | Yes (Ser 32) | Set 1 | W1201 | Download |
|
JNK (Human) |
JNK | No | Yes (Thr 183/Tyr 185) | Set 1 | W1201 | Download |
|
p65 (Human) |
NF-κB |
Yes | Yes (Ser 536) | Set 2 | W1331 | Download |
|
Rb (Retinoblastoma Tumor Suppressor - Human) |
Cell Cycle | Yes | Yes (Ser 780) | Set 2 | W1331 | Download |
|
Rb (Retinoblastoma Tumor Suppressor - Human) |
Cell Cycle | Yes | Yes (Ser 807/811) | Set 2 | W1331 | Download |
|
Smad1 (Human) |
BMP | Yes | Yes (Ser 463/465) | Set 1 | W1201 | Download |
|
Smad2 (Human) |
TGF-β | Yes | Yes (Ser 465/467) | Set 2 | W1331 | Download |
|
STAT1 (Human) |
JAK/STAT | Yes | Yes (Tyr 701) | Set 1 | W1201 | Download |
|
STAT1 (Human) |
JAK/STAT | Yes | Yes (Ser 727) | Set 1 | W1201 | Download |
|
STAT2 (Human) |
JAK/STAT | No | Yes (Tyr 690) | Set 1 | W1201 | Download |
|
STAT 3 (Human) |
JAK/STAT | Yes | Yes (Tyr 705) | Set 1 | W1201 | Download |
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